c chemokine receptor type 7  (Novus Biologicals)

Journal: International Journal of Molecular Medicine

Article Title: Prostaglandin E2 serves a dual role in regulating the migration of dendritic cells

doi: 10.3892/ijmm.2020.4801

Figure Lengend Snippet: Effect of PGE 2 on the expression of surface molecules of DCs determined by flow-cytometry. The numbers at the upper right corner of each plot indicate the percentage of positive cells. (A) Expression of costimulatory markers (CD40, CD80, CD86 and MHC II) on DCs. (B) CCR7 expression on DCs. Bar charts demonstrating the expression of (C) costimulatory markers (CD40, CD80, CD86 and MHC II) and (D) CCR7 on DCs. Data are representative of three independent experiments and are presented as the mean ± SEM. * P<0.05 vs. 0 µ g/ml PGE 2 . DC, dendritic cell; PGE 2 , prostaglandin E2; MHC, major histocompatibility complex; CCR7, CC chemokine receptor type 7.

Article Snippet: The antibodies used included phycoerythrin-conjugated anti-mouse CD40 (1:100; cat. no. 12-0401), CD80 (1:100; cat. no. 12-0801), CD86 (1:100; cat. no. 12-0862), major histocompatibility complex II (MHCII; 1:100; cat. no. 12-5321) and C-C chemokine receptor type 7 (CCR7; 1:100; cat. no. 12-1971; all from eBioscience; Thermo Fisher Scientific, Inc.).

Techniques: Expressing, Flow Cytometry, Immunopeptidomics

Journal: International Journal of Molecular Medicine

Article Title: Prostaglandin E2 serves a dual role in regulating the migration of dendritic cells

doi: 10.3892/ijmm.2020.4801

Figure Lengend Snippet: Effect of PGE 2 on the expression of surface molecules of DCs determined by flow-cytometry. The numbers at the upper right corner of each plot indicate the percentage of positive cells. (A) Expression of costimulatory markers (CD40, CD80, CD86 and MHC II) on DCs. (B) CCR7 expression on DCs. Bar charts demonstrating the expression of (C) costimulatory markers (CD40, CD80, CD86 and MHC II) and (D) CCR7 on DCs. Data are representative of three independent experiments and are presented as the mean ± SEM. * P<0.05 vs. 0 µ g/ml PGE 2 . DC, dendritic cell; PGE 2 , prostaglandin E2; MHC, major histocompatibility complex; CCR7, CC chemokine receptor type 7.

Article Snippet: The antibodies used included phycoerythrin-conjugated anti-mouse CD40 (1:100; cat. no. 12-0401), CD80 (1:100; cat. no. 12-0801), CD86 (1:100; cat. no. 12-0862), major histocompatibility complex II (MHCII; 1:100; cat. no. 12-5321) and C-C chemokine receptor type 7 (CCR7; 1:100; cat. no. 12-1971; all from eBioscience; Thermo Fisher Scientific, Inc.).

Techniques: Expressing, Flow Cytometry